Ro 90-7501 Is a Novel Radiosensitizer for Cervical Cancer Cells that Inhibits ATM Phosphorylation
Background/Aim: Ro 90-7501 has been identified as an inhibitor of amyloid β42 fibril formation, a hallmark of Alzheimer’s disease. This study aimed to investigate its potential radiosensitizing effects, with a focus on ATM signaling following irradiation.
Materials and Methods: HeLa cells were treated with irradiation and Ro 90-7501, followed by clonogenic survival, apoptosis, and cell cycle analyses, as well as western blotting. A tumor growth delay assay was conducted in BALB/c-nu mice.
Results: Ro 90-7501 enhanced the effects of irradiation, significantly CCT241533 reducing clonogenic survival and delaying tumor growth. It also increased apoptosis and disrupted cell cycle progression post-irradiation. Western blot analysis revealed that Ro 90-7501 suppressed the phosphorylation of ATM and its downstream targets, including H2AX, Chk1, and Chk2.
Conclusion: Ro 90-7501 exerts a radiosensitizing effect on cervical cancer cells by inhibiting ATM-mediated DNA damage response pathways.